Analyzers
The RFCC at Cincinnati Children's maintains ten analytical cytometers for measuring fluorescence in cellular applications including immunofluorescence, cell cycle analysis, proliferation and phospho-flow. The instruments are available 24/7 for those who have been trained by our staff.
Design a panel on one of our instruments using FluoroFinder.
| Instrument Name* | Instrument Make/Model** | Location*** | UV (355nm) | Violet (405nm) | Blue (488nm) | Yellow-Green (561nm) | Red (635nm) |
| Canto 1 | BD/FACSCanto | R5087A | 2 |
4 | 3 |
||
| Canto 2 | BD/FACSCanto II | R5087A | 2 | 4 | 2 |
||
| Canto 3 | BD/FACSCanto | R5087A | 3 | 5 |
3 |
||
| Fortessa 1 | BD/LSRFortessa | R5524 | 2 | 6 |
2 |
5 | 3 |
| Fortessa 2 | BD/LSRFortessa | R5524 | 2 | 6 |
2 |
5 | 3 |
| Fortessa 3 |
BD/LSRFortessa | R5524 | 2 | 6 |
2 |
5 | 3 |
| A5 | BD/FACSymphony A5 | R5524 | 14 | 9 | 9 | 6 | 3 |
| Aurora | Cytek/Aurora | R5509 | 16 |
16 |
14 |
10 |
8 |
| Maleficent | Cytek/Aurora | R5509 | 16 |
16 |
14 |
10 |
8 |
| Diablo | Cytek/Northern Lights | R5509 | 16 | 14 | 8 | ||
|
Analyzer Information |
|
Location Hours Contact Us To Schedule Time |
*Click on the instrument name for the configuration.
**Only the following BD Falcon tubes can be used on our instruments. BD Cat. No. 352058, 352052, 352008, 352235. Any other tube will damage the instrument!
***BL2 rooms. Doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.
Scheduling
After training, you can schedule time using the Stratocore Scheduling system. Times of more than four hours must be approved by flow core staff. Schedule only for the time you need and delete any unused time.
Sample Transportation to the RFCF
All samples must be placed in sealed capped tubes and in a secondary leak-proof container with absorbent. Please refer to the Transporting Samples to the RFCF document for more details.
Cancellation Policies and Fees
Pricing for internal clients can be found on Centerlink. For external clients, please contact us. See policies for more details about other fees. You need to cancel your appointment via Stratocore. All cancellations will be confirmed via email to the client.
Technical Support
This video provides insight on how to import/export templates on FACSDiva.
Please look at this troubleshooting document for basic troubleshooting of the BD instruments.
Look at those resources for more information on Spectral Flow Cytometry
- Cytek resources
- Panel design: SpectroLearn with troubleshooting advice
- Overview of the software SpectroFlo
- Create and unmix an experiment in SpectroFlo
- Worksheet tool
- Batch analysis
- Area Scaling
- University of Chicago resources
Need help? We can be reached in R5503, R5564 or at 513-636-3575
Data Storage
A project drive has been set up called “RFCC-FACSDATA.” For the Aurora the drive is called "RFCC-AURORA". Directions on how to access the project drive will be provided at training or refer to our data management policy for further information.
Request access to one of our project drives (Cincinnati Children's users only) at https://bmirdsdp/DP.
Data Analysis
Any time spent on the acquisition computers is considered billable time. However, computers are available for data analysis free of charge to flow core users in R5503. See "Software" for more information.
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