Exploring Lipid Subspecies Functions
LDL is thought to contribute to heart disease or the development of atherosclerosis while HDL is thought to prevent against atherosclerosis. Our lab is interested in how LDL (the lipoprotein) gets into the blood vessel wall and starts the initial steps of atherosclerosis, and how HDL may prevent this. We are also interested in how type 2 diabetes affects these steps. We use in vivo experiments, in vitro assays, and surface plasmon resonance to study these pathways.
Funding
This work is funded through research grants from the NIH and in part by Cincinnati Children's Hospital Medical Center.
Publications from this Study
Bedi, S; Morris, J; Shah, AS; Hart, RC; Jerome, WG; Aller, SG; Tang, C; Vaisar, T; Bornfeldt, KE; Segrest, JP; Heinecke, JW; Davidson, WS. Conformational Flexibility of Apolipoprotein A-I amino- and carboxy-Termini is necessary for Lipid Binding but not Cholesterol Efflux. J Lipid Res. 2022 Jan 17:100168.
Davidson, WS; Shah, AS; Sexmith, H; Gordon, S. The HDL Proteome Watch: Compilation of studies leads to new insights on HDL function. Biochim Biophys Acta Mol Cell Biol Lipids. 2021 Nov 18;1867(2):159072.
Swertfeger, DK; Rebholz, S; Li, H; Shah, AS; Davidson, WS; Long, JL. Feasibility of a Plasma Bioassay to Assess Oxidative Protection of Low Density Lipoproteins by High Density Lipoproteins. J Clin Lipidol. 2018 Nov - Dec;12(6):1539-1548.
Cooke, AL; Morris, J; Melchior, JT; Street, SE; Jerome, WJ; Huang, R; Herr, A; Smith, LE; Segrest, JP; Remaley, AT; Shah, AS; Thompson, TB; Davidson, WS. A Thumbwheel Mechanism for Modulation of Lecithin:cholesterol Acyl Transferase Activity in HDL. J Lipid Res. 2018 Jul;59(7):1244-1255.
Melchior, JT; Street, SE; Andraski, AB; Furtado, JD; Sacks, FM; Shute, RL; Greve, EI; Swertfeger, DK; Li, H; Shah, AS; Lu, JL; Davidson, WS. Apolipoprotein A-II alters the proteome of human lipoproteins and enhances cholesterol efflux from ABCA1. J Lipid Res. 2017 Jul;58(7):1374-1385.
Swertfeger, DK; Li, H; Rebholz, S; Zhu, X; Shah, AS; Davidson, WS; Lu, JL. Mapping Atheroprotective Functions and Related Proteins/Lipoproteins in Size Fractionated Human Plasma. Mol Cell Proteomics. 2017 Apr;16(4):680-693.
Gordon, SM; Li, H; Zhu, X; Tso, P; Reardon, CA; Shah, AS; Lu, JL; Davidson, WS. Impact of Genetic Deletion of Platform Apolipoproteins on the Size Distribution of the Murine Lipoproteome. J Proteomics. 2016 Sep 2;146:184-94.
Davidson, WS; Heink, A; Sexmith, H; Melchior, JT; Gordon, SM; Kuklenyik, Z; Woolett, L; Barr, JR; Jones, JI; Toth, CA; Shah, AS. The Effects of Apolipoprotein B Depletion on HDL Subspecies Composition and Function. J Lipid Res. 2016 Apr;57(4):674-86.
Heink, A; Davidson, WS; Swertfeger, D; Lu, JL; Shah, AS. A Comparison of Methods to Enhance Protein Detection of Lipoproteins by Mass Spectrometry. J Proteome Res. 2015 Jul 2;14(7):2943-50.
Gordon, SM; Li, H; Zhu, X; Shah, AS; Lu, LJ; Davidson, WS. A Comparison of the Mouse and Human Lipoproteome: Suitability of the Mouse Model for Studies of Human Lipoproteins. J Proteome Res. 2015 Jun 5;14(6):2686-95.
Gordon, SM; Deng, J; Tomann, AB; Shah, AS; Lu, JL; Davidson, WS. Multi-dimensional co-separation analysis reveals protein:protein interactions defining plasma lipoprotein subspecies. Mol Cell Proteomics. 2013 Jul 23.
Shah, AS; Tan, L; Lu, JL; Davidson, WS. The proteomic diversity of high density lipoproteins: Our emerging understanding of its importance in lipid transport and beyond. J Lipid Res. 2013 Oct;54(10):2575-85.
