Research Flow Cytometry Facility
Software

Software Available to Internal and External Scientists

Analysis Workstations

Analysis workstations are available, or users can purchase software licenses for data analysis. See below for a listing of available software packages.

Analysis Workstation	Location	Analysis Software Programs
PC1	R5503	FACSDiva, FlowJo
PC2	R5503	FACSDiva, FlowJo, FCSExpress, ModFit LT
PC3	R5509	SpectroFlo (Aurora), FlowJo
PC4	R5503	IDEAS with Machine Learning Module, Cell Sorter (SH800S and MA900)
PC5	R5509	SpectroFlo (Maleficent), FlowJo

The Research Flow Cytometry Facility also hosts site licenses for FlowJo. To sign up for a FlowJo license, see instructions below. For more information about FCSExpress, Cytobank and high dimensional analysis, please email Celine Silva Lages.

Software Information:

FACSDiVa (BD Biosciences). Watch a video overview of FACSDiva
FCSExpress (DeNovo Software). Visit DeNovo Software website for technical support
FlowJo(BD Biosciences). See the online manual, the tutorial, and the basic and advanced training video recorded on June 15 and 16, 2020 for Cincinnati Children's on FlowJo v10.6. Resources available for Intro to FlowJo Workshop and FlowJo High Parameter Workshop at Cincinnati Children's on February 8 and 9, 2022.
ModFit LT (Verity Software House). Watch video tutorials
ImageStream | Cytek Biosciences (Cytek). Visit ImageStream | Cytek Biosciences for more information.
SpectroFlo (Cytek). Visit the Cytek support page for more information.
Cell Sorter SH800S and MA900 (SONY). Visit the SONY support page for more information.

Site Licenses for FlowJo

The Research Flow Cytometry Facility hosts a site license for FlowJo flow cytometry analysis software. See the table below for information about the license, pricing structure and how to participate.

FAQs

FAQ	FlowJo
Who can participate?	Research Flow Cytometry Facility users from Cincinnati Children's and UC can participate.
Is it MAC or PC compatible?	Compatible with both Mac and PC platforms
What is the pricing structure?	Licensed to each user. Four computers per license.
What if I already have it?	Contact us and we will work with the company to include you in the license.
How do I sign up?	1.Fill out the license agreement and return it to the facility. 2.Create your portal ID by following the link in an email sent by FlowJo. 3.Download the last version of FlowJo. 4.Login to FlowJo with your portal ID.
Can I use it on multiple computers?	Yes. Each FlowJo license is specific to one user. You can use the license on up to four computers, one at a time.
Can I have multiple users on one license?	No. The site license is specific to each user by creating a unique login.

Location and Contact Information

Location: Burnet Campus, R Building, 5th Floor. See room details in the above chart.

Hours: Monday through Friday, 9 am to 5 pm

Contact, Fees and Scheduling: For more information about our equipment, services, training and pricing or to begin working with us, please contact us.

Our Response to the IV Fluid Shortage

Research Flow Cytometry FacilitySoftware