Cincinnati Center for Eosinophilic Disorders Research
Eosinophilic Gastrointestinal Disorder Diagnostics

Eosinophilic Gastrointestinal Disorder Diagnostics

Our research integrates multidisciplinary basic studies, with particular emphasis on modern genetic approaches, translational research employing novel in vivo and ex vivo systems developed by our laboratory and clinical studies designed to test proof-of-concept theories and novel therapeutics directly in humans.

We strive to translate our research into clinical utility and practice, as exemplified by our development of diagnostics for eosinophilic gastrointestinal disorders.

Our Tests

 

Test

 

Description

 

Reference

 

Contact

 
Esophageal Peak Eosinophil Count ≥15 eosinophils / high-power microscopic field and clinical symptoms Furuta et al. Gastroenterology. 2007

Liacouras et al. J Allergy Clin Immunol. 2011
Margaret H. Collins, MD
Professor of Pathology & Laboratory Medicine
Margaret.collins@cchmc.org
Eosinophilic Esophagitis Diagnostic Panel (EDP; clinically available as EoGenius(TM) from Miraca Life Sciences) Next-generation molecular diagnostic with high accuracy for eosinophilic esophagitis Wen et al. Gastroenterology. 2013

Butz et al. Gastroenterology. 2014
Marc E. Rothenberg, MD, PhD
Professor of Pediatrics

Rothenberg@cchmc.org

Pediatric Eosinophilic Esophagitis Symptom Severity Module, version 2.0 (PEESS v2.0)

 
Symptom severity scoring metric for patients with eosinophilic esophagitis

Franciosi et al. BMC Gastroenterology. 2011

Martin et al. J Allergy Clin Immunol. 2015

Lisa J. Martin, PhD

Professor of Human Genetics lisa.martin@cchmc.org

 

Test

 

Description

 

Reference

 

Contact

 
Proton pump inhibitor (PPI)-Responsive Esophageal Eosinophilia (PPI-REE) Prediction Panel Next-generation molecular diagnostic panel (e.g. KCNJ2) Wen et al. J Allergy Clin Immunol. 2015 Marc E. Rothenberg, MD, PhD
Professor of Pediatrics
Rothenberg@cchmc.org
Esophageal microRNA Panel

MicroRNA panel of esophageal biopsy Lu et al. J Allergy Clin Immunol. 2012 Marc E. Rothenberg, MD, PhD
Professor of Pediatrics
Rothenberg@cchmc.org
Histology Scoring System (HSS) An extended analysis of histologic pathology including score and grade Collins et al. J Allergy Clin Immunol. 2012

 

Collins et al. Dis Esophagus. 2016

Margaret H. Collins, MD
Professor of Pathology & Laboratory Medicine
Margaret.Collins@cchmc.org

Serum Cytokines

Panel of serum proteins

Konikoff et al. Clin Gastroenterol Hepatol. 2006

Blanchard et al. J Allergy Clin Immunol. 2011

Marc E. Rothenberg, MD, PhD
Professor of Pediatrics
Rothenberg@cchmc.org

Circulating microRNAs

Panel of serum microRNAs

Lu et al. J Allergy Clin Immunol. 2012

Marc E. Rothenberg, MD, PhD
Professor of Pediatrics
Rothenberg@cchmc.org

Rapid Biopsy Diagnostic

Same-day polychromatic FACS

Wen et al. J Allergy Clin Immunol. 2017 Marc E. Rothenberg, MD, PhD
Professor of Pediatrics
Rothenberg@cchmc.org

Eosinophilic Gastritis Panel

Next-generation molecular diagnostic

Caldwell et al. J Allergy Clin Immunol. 2014

Marc E. Rothenberg, MD, PhD
Professor of Pediatrics
Rothenberg@cchmc.org

Genetic Propensity Panel

Single-nucleotide variant analysis and/or whole-exome sequencing

Rothenberg et al. Nature Genetics. 2010

Kottyan et al. Nature Genetics. 2014

Marc E. Rothenberg, MD, PhD
Professor of Pediatrics
Rothenberg@cchmc.org

Eosinophil Progenitor (EoP) Cell Level

Measure levels of EoP in the blood

Morris et al. J Allergy Clin Immunol. 2016

Patricia C. Fulkerson, MD, PhD
Assistant Professor of Pediatrics

EoE Diagnostic Panel

EoE Diagnostic Panel
The Rothenberg Lab developed a molecular diagnostic test called the eosinophilic esophagitis diagnostic panel (EDP). This test uses low-density Taqman quantitative PCR array of biopsy tissue to distinguish patients with eosinophilic esophagitis, the disease activity, and exposure to swallowed glucocorticoids. This test is now clinically available as EoGenius™. Read More

PPI-Responsive Esophageal Eosinophilia

PPI-Responsive Esophageal Eosinophilia
Esophageal eosinophilia can be resistant or responsive to proton pump inhibitors (PPI), representing eosinophilic esophagitis (EoE) if it is resistant and PPI-responsive esophageal eosinophilia (PPI-REE) if it is responsive. A recent, collaborative study by the Wen Lab and Rothenberg Lab shows significant molecular overlap between EoE and PPI-REE transcriptomes, suggesting that these conditions have similar underlying mechanisms. Read More