Cell Sorting
The RFCF at Cincinnati Children’s operates six cell sorters for the simultaneous purification of up to six populations to be used for downstream applications. If you are a new client, please make sure you have a Stratocore account, an IBC protocol, and take the required courses (Laser Safety- Research Flow Cytometry Facility, RFCC Course 1 and Course 10 can be found on the training tab). Once courses are completed, users must submit a "Cell Sorter Training/Consultation Form" under the "Request" tab in Stratocore to schedule a consultation.
Users can be trained on the Sonys (SH800S and MA900), FACSArias, FACSymphony S6, and the Bigfoot. Those instruments are available 24/7 for user operated sorts of up to two, four and six populations respectively.
Design a panel on one of our instruments using FluoroFinder.
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Number of Fluorescence PMTs |
Number of cell population |
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| Instrument Name* | Instrument Make/Model | Location** | UV (335nm) | Violet (405nm) | Blue (488nm) | Yellow-Green (561nm) | Red (635nm) | |
| Fusion | BD/FACSAria II/Fusion | R5503 | 2 | 6 | 2 |
5 |
3 |
4 |
| S6 | BD/FACSymphony S6 | R5503 | 8 | 8 |
6 |
5 | 3 |
6 |
| S6 SE | BD/FACSymphony S6 | R5503 | 10 | 14 | 9 | 9 | 6 | 6 |
| SH800S | Sony/SH800S | R5504 | 4 collinear lasers, 6 Channels |
2 |
||||
| MA900 | Sony/MA900 | R5504 | 2 set of 2 collinear lasers, 12 channels |
4 |
||||
| Bigfoot | ThermoFisher/Bigfoot | R5529 | 12 |
12 |
7 |
12 |
5 |
6 |
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Cell Sorting Information |
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Location Hours Room phone numbers |
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*Click on the instrument name for the configuration.
**BL2 rooms. Doors must be kept closed at all times and appropriate PPE worn while in the instrument rooms.
Collection options include: 1.5 ml tubes; 12x75 mm Falcon tubes; 15 ml conicals; multiwell plates; slides.
Scheduling
After your sorting consultation, you can schedule time using Stratocore. Please put a request at least 24 hours, during working days, before your needed time for approval.
To schedule the right amount of time for staff-operated sort, please refer to the time estimate document for staff-assisted sorts.
Sample Transportation to the RFCF
All samples must be placed in sealed capped tubes and in a secondary leak-proof container with absorbent. Please refer to the Transporting Samples to the RFCF document for more details.
Sample preparation
Read the Cell Sorting Guidelines for information on sample preparation, collection, etc.
Sorting fees
The sorting fees for internal clients can be found on Centerlink. For external clients, please contact us.
Cancellation / late policy
You need to cancel your appointment via Stratocore. All cancellations will be confirmed via email to the client. You will be charged for the sort and the setup fee if you cancel less than 24 hours before the scheduled time. For Monday sorts, you must cancel by noon on Friday. If you are late, you will be charged for the time you scheduled. If your sort time runs over, you will be charged for the time you used. See policies for more details.
Infectious cell sorting
All of our cell sorters are contained in class II biosafety cabinets for sorting of potentially infectious samples. Contact Celine Silva Lages for more information on infectious cell sorting. Please see this list for a non-exhaustive list of BSL2 and BSL2+ samples.
After your sort
Tell us how we’re doing! After your appointment, fill out a survey to provide feedback on your sort.
