GLI activator and repressor activity have differential requirements in the NCCs of the developing mandibular prominence. (A, D, G, J) H&E staining on frontal sections of wild-type, Kif3af/f;Wnt1-Cre, Gli2f/f;Gli3d699;Wnt1-Cre, and ROSAGli3TFlag;Wnt1-Cre embryos. Dotted black lines indicate developing and mutant submandibular glands (SMGs). (A’, D’, G’, J’) High mag images of A, D, G, J. (B, E, H, K) Anti-PDGFRβ immunostaining (green) on wild-type, Kif3af/f;Wnt1-Cre, Gli2f/f;Gli3d699;Wnt1-Cre, and ROSAGli3TFlag;Wnt1-Cre SMGs or mutant SMG mesenchymal capsules (dotted white lines). (C, F, I, L) Anti-Krt8 immunostaining (red) in wild-type, Kif3af/f;Wnt1-Cre, Gli2f/f;Gli3d699;Wnt1-Cre, and ROSAGli3TFlag;Wnt1-Cre SMGs or mutant SMG mesenchymal capsules (dotted white lines). Nuclei counterstained with Hoechst.