Sequencing and Analysis Techniques and Services

Techniques

The MGML offers microbiome sequencing and analysis including:

Metagenomic Sequencing

• DNA and RNA extraction
• Shotgun DNA sequencing
• RNA sequencing, including host and microbial RNA
• 165 rRNA amplicon sequencing

Bioinformatic Analysis to determine:

• Taxonomic composition and differential microbial abundance
• Metabolic functional gene assignment and differential abundance
• RNA sequence with assignment to microbial and human gene databases for differential transcript abundance

Microbial Genome Sequencing and Analysis

• Comparison of genome similarity (molecular epidemiology)
• Virulence gene and antibiotic resistance gene detection and analysis 

Services

The MGML offers use of several sequencing and analysis kits: