Helmrath Lab

Publications

Sawada, K; Oates, JR; Kimura, M; Stankiewicz, TE; Eom, J; Alarcon, PC; Chung, H; Damen, MSMA; Ulanowicz, CJ; Wayland, JL; Helmrath, MA; Takebe, T; Moreno-Fernandez, ME; Divanovic, S. Glutamine metabolism as a rheostat of CD4+ T cell pathogenic function in metabolic dysfunction-associated steatotic liver disease. Cell Metabolism. 2026.

Kuo, CJ; Blutt, S; Donowitz, M; Estes, MK; Guha, C; Klein, OD; Li, L; Niland, JC; Santos, AJM; Shivdasani, RA; Wang, TC; Yan, KS; Zachos, NC; Helmrath, MA. Building and regenerating intestines by manipulating intestinal stem cells. Nature Reviews Gastroenterology and Hepatology. 2026.

Poling, HM; Singh, A; Kongsomros, S; Sheth, M; Krutko, M; Reza, AA; Srivastava, K; Wells, JM; Helmrath, MA; Esfandiari, L. Promoting Human Intestinal Organoid Formation and Stimulation Using Piezoelectric Nanofiber Matrices. Tissue Engineering - Part A.. 2026; 32(17-18):604-615.

Yodoshi, T; Ollberding, NJ; Mcneill, M; Ley, SL; Kirk, S; Kleiner, DE; Arce-Clachar, AC; Bramlage, K; Inge, TH; Helmrath, MA; Cecil, KM; Trout, AT; Elder, DA; Mouzaki, M; Xanthakos, SA. Vertical Sleeve Gastrectomy Versus Comprehensive Lifestyle Intervention for Adolescents With Metabolic Dysfunction-Associated Steatotic Liver Disease: A Prospective Controlled Cohort Study. Alimentary Pharmacology and Therapeutics. 2026; 64(6):758-769.

Segev, N; Nadeau, KJ; Kelsey, M; Schaaf, LR; Baumgartner, A; Riegler, A; Wiitala, K; Jenkins, TM; Xanthakos, SA; Helmrath, MA; Inge, TH; Shah, AS. Glycemic Normalization Immediately Following Vertical Sleeve Gastrectomy in Adolescents With Type 2 Diabetes From the ST2OMP Study. Diabetes, Obesity and Metabolism. 2026; 28(8):7555-7561.

Kittle, HD; Bergus, KC; Bricker, J; Fallat, M; Hertweck, SP; Hewitt, G; Lutz, C; Aldrink, JH; Bence, C; Breech, L; St Peter, SD; Asti, L; Deans, K; Minneci, PC. Use of Radiographic Features for Risk Stratification of Ovarian Masses in Children and Adolescents. Journal of Surgical Research. 2026; 326:507-517.

Goddard, GR; Oliveira, S; Slaughter, C; Klotz, K; Stoops, M; Schletker, J; Wessel, J; Helmrath, M; Kocoshis, S; Goldschmidt, M; Wales, PW. Care transition from a pediatric intestinal rehabilitation program to adult care and the risk of all-cause mortality: A retrospective cohort study. Journal of Parenteral and Enteral Nutrition. 2026; 50(5):677-683.

Poling, HM; Noël, T; Singh, A; Fisher, GW; Thorner, K; Chaturvedi, P; Nattamai, K; Srivastava, K; Batie, MR; Hausfeld, T; Takebe, T; Wells, JM; Helmrath, MA; Mahe, MM. Large-scale and innervated functional human gut tissues for transplantation via transient spheroid confinement. Nature Biomedical Engineering. 2026.

Moran-Lev, H; Kocoshis, SA; Mukasa, M; Oliveira, SB; Helmrath, MA; Wales, PW; Cole, CR. Contemporary challenges and outcomes in children with ultrashort bowel syndrome: A descriptive cohort study. Journal of Pediatric Gastroenterology and Nutrition. 2026; 82(5):1296-1302.

Beamish, AJ; Jenkins, T; Ryder, JR; Courcoulas, A; Dorman, RM; Michalsky, M; Harmon, C; Helmrath, M; Inge, TH. Healthcare utilisation across five years among adolescents following metabolic-bariatric surgery; a prospective observational study. The Lancet Regional Health. Americas. 2026; 54:101332.

Turpin, AG; Asti, L; Lutz, C; Saito, JM; Mak, GZ; Hirschl, RB; Gadepalli, S; Helmrath, MA; Leys, CM; Lal, DR; Kabre, R; Fallat, ME; Deans, KJ; Minneci, PC. Is Appendiceal Cancer a Risk of Nonoperative Management of Pediatric Uncomplicated Appendicitis? Journal of Surgical Research. 2026; 317:161-166.

Mukasa, M; Moran-Lev, H; Hornung, L; Kocoshis, SA; Oliveira, SB; Helmrath, MA; Wales, PW; Cole, CR. Clinical outcomes of teduglutide therapy in children with short bowel syndrome-associated intestinal failure: A single-center experience. Intestinal Failure. 2026; 11:100382.

Damen, MS; Alarcon, PC; Chan, CC; Stankiewicz, TE; Chung, H; Sawada, K; Ulanowicz, CJ; Eom, J; Oates, JR; Wayland, JL; Helmrath, MA; Sanchez-Gurmaches, J; Moreno-Fernandez, ME; Divanovic, S. A shift in PKM2 oligomeric state instructs adipocyte inflammatory potential. JCI Insight. 2025; 10(22).

Kim, A; Okura, T; Choi, K; Gill, R; Borra, VJ; Murakami, K; Poulos, A; Zhang, X; Jenkins, T; Shah, AS; Helmrath, M; Nakamura, T. Exploring Organ-Specific Extracellular Vesicles in Metabolic Improvements Following Bariatric Surgery in Youth With Obesity. Obesity. 2025; 33(11):2181-2194.

Soussi, FEA; Brusilovsky, M; Buck, E; Bacon, WC; Dadgar, S; Fullerton, A; Durban, VM; Barrile, R; Helmrath, MA; Takebe, T; Roth, A; Kasendra, M. Autologous Organoid-T Cell Co-Culture Platform for Modeling of Immune-Mediated Drug-Induced Liver Injury. Advanced Science. 2025; 12(43):e08584.

Lin, AM; Bergus, KC; Bricker, J; Fallat, M; Hertweck, SP; Hewitt, G; Lutz, C; Aldrink, JH; Bence, C; Breech, L; St Peter, SD; Asti, L; Deans, K; Minneci, PC. Use of Ovarian Tumor Markers in Prediction of Malignancy Risk in Pediatric and Adolescent Patients. Journal of Surgical Research. 2025; 315:839-846.

Miao, Y; Pek, NM; Tan, C; Jiang, C; Yu, Z; Iwasawa, K; Shi, M; Kechele, DO; Sundaram, N; Pastrana-Gomez, V; Zorn, AM; Chen, Y-W; Guo, M; Gu, M. Co-development of mesoderm and endoderm enables organotypic vascularization in lung and gut organoids. Cell. 2025; 188(16):4295-4313.e27.

Oliveira, SB; Thomas, JD; Cole, C; Helmrath, M; Kocoshis, S; Wales, PW. Parenteral nutrition weaning in pediatric intestinal failure patients enrolled in remote patient monitoring: A descriptive study. Journal of Parenteral and Enteral Nutrition. 2025; 49(6):768-772.

Krutko, M; Poling, HM; Sheth, M; Kongsomros, S; Bryan, AE; Sharma, M; Singh, A; Reza, HA; Wikenheiser-Brokamp, KA; Takebe, T; Helmrath, MA; Harris, GM; Esfandiari, L. Thermal Annealing Enhances Piezoelectricity and Regenerative Potential of PVDF-TrFE Nanofiber Scaffolds. Advanced Materials Technologies. 2025.

Shi, M; Crouse, B; Sundaram, N; Pode Shakked, N; Thorner, K; King, NM; Dutta, P; Ester, L; Zhang, W; Govindarajah, V; Mayhew, CN; Helmrath, MA; Bonventre, JV; Mccracken, KW. Integrating collecting systems in human kidney organoids through fusion of distal nephron to ureteric bud. Cell Stem Cell. 2025; 32(7):1055-1070.e8.

Thivalapill, N; Jenkins, TM; Inge, TH; Xie, C; Courcoulas, AP; Harmon, CM; Helmrath, MA; Sisley, S; Michalsky, MP; Ryder, JR. Functional mobility and pain are improved for 6 years after adolescent bariatric surgery. Obesity. 2025; 33(6):1126-1135.

Childs, CJ; Poling, HM; Chen, K; Tsai, Y-H; Wu, A; Vallie, A; Eiken, MK; Huang, S; Sweet, CW; Schreiner, R; Helmrath, MA; Walton, KD; Rafii, S; Spence, JR. Coordinated differentiation of human intestinal organoids with functional enteric neurons and vasculature. Cell Stem Cell. 2025; 32(4):640-651.e9.

Mulero-Russe, A; Mora-Boza, A; Marquez, EN; Ziegelski, M; Helmrath, M; García, AJ. Synthetic hydrogel substrate for human induced pluripotent stem cell definitive endoderm differentiation. Biomaterials. 2025; 315:122920.

Gil, LA; Asti, L; Chen, H-F; Saito, JM; Pattisapu, P; Deans, KJ; Minneci, PC. Cost-Effectiveness of Nonoperative Management vs Upfront Laparoscopic Appendectomy for Pediatric Uncomplicated Appendicitis for 1 Year. Journal of the American College of Surgeons. 2025; 240(3):288-298.

Jurickova, I; Dreskin, BW; Angerman, E; Bonkowski, E; Nguyen, J; Villarreal, R; Tominaga, K; Iwasawa, K; Braun, T; Takebe, T; Helmrath, MA; Haberman, Y; Wells, JM; Denson, LA. Eicosatetraynoic Acid Regulates Profibrotic Pathways in an Induced Pluripotent Stem Cell-Derived Macrophage-Human Intestinal Organoid Model of Crohn's Disease. Journal of Crohn's and Colitis. 2025; 19(2).

Moran-Lev, H; Kocoshis, SA; Córdova Amador, I; Mukasa, M; Oliveira, SB; Helmrath, M; Cole, CR. Factors associated with accelerated parenteral weaning in children with intestinal failure: A descriptive cohort study. Journal of Parenteral and Enteral Nutrition. 2025; 49(2):207-213.

Tominaga, K; Kechele, DO; Sanchez, JG; Vales, S; Jurickova, I; Roman, L; Asai, A; Enriquez, JR; Mccauley, HA; Kishimoto, K; Zorn, AM; Helmrath, MA; Denson, LA; Wells, JM. Deriving Human Intestinal Organoids with Functional Tissue-Resident Macrophages All From Pluripotent Stem Cells. Cellular and Molecular Gastroenterology and Hepatology (CMGH). 2025; 19(4):101444.

Ryder, JR; Jenkins, TM; Xie, C; Courcoulas, AP; Harmon, CM; Helmrath, MA; Sisley, S; Michalsky, MP; Brandt, M; Inge, TH. Ten-Year Outcomes after Bariatric Surgery in Adolescents. New England Journal of Medicine. 2024; 391(17):1656-1658.

Poling, HM; Sundaram, N; Fisher, GW; Singh, A; Shiley, JR; Nattamai, K; Govindarajah, V; Cortez, AR; Krutko, MO; Ménoret, S; Mayhew, CN; Takebe, T; Mahe, MM; Helmrath, MA. Human pluripotent stem cell-derived organoids repair damaged bowel in vivo. Cell Stem Cell. 2024; 31(10):1513-1523.e7.

Poling, HM; Brown, N; Wells, JM; Barrile, R; Helmrath, MA; Mahe, MM. Mechanical stimulation promotes human intestinal villus morphogenesis in vivo. Biophys Rev (Melville). 2024; 5(3):032102.

Speer, AL; Lally, KP; Pedroza, C; Zhang, Y; Poindexter, BB; Chwals, WJ; Hintz, SR; Besner, GE; Stevenson, DK; Ohls, RK; Rysavy, MA; Das, A; Tyson, JE; Blakely, ML. Surgical Necrotizing Enterocolitis and Spontaneous Intestinal Perforation Lead to Severe Growth Failure in Infants. Annals of Surgery. 2024; 280(3):432-443.

Swertfeger, D; Kim, A; Sexmith, H; Moreno-Fernandez, ME; Davidson, WS; Helmrath, M; Jenkins, T; Okura, T; Geh, E; Xanthakos, SA; Szabo, S; Nakamura, T; Divanovic, S; Shah, AS. Presurgery health influences outcomes following vertical sleeve gastrectomy in adolescents. Obesity. 2024; 32(6):1187-1197.

O'connell, AE; Raveenthiraraj, S; Oliveira, LFS; Adegboye, C; Dasuri, VS; Qi, W; Khetani, RS; Singh, A; Sundaram, N; Lin, J; Turner, JR; Agrawal, PB; Helmrath, M; Breault, DT. WNT2B Deficiency Causes Enhanced Susceptibility to Colitis Due to Increased Inflammatory Cytokine Production. Cellular and Molecular Gastroenterology and Hepatology (CMGH). 2024; 18(2):101349.

Pode-Shakked, N; Slack, M; Sundaram, N; Schreiber, R; Mccracken, KW; Dekel, B; Helmrath, M; Kopan, R. RAAS-deficient organoids indicate delayed angiogenesis as a possible cause for autosomal recessive renal tubular dysgenesis. Nature Communications. 2023; 14(1):8159.

Múnera, JO; Kechele, DO; Bouffi, C; Qu, N; Jing, R; Maity, P; Enriquez, JR; Han, L; Campbell, I; Mahe, MM; Hu, Y-C; Takebe, T; Helmrath, MA; Wells, JM. Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon. Cell Stem Cell. 2023; 30(11):1434-1451.e9.

Minneci, PC; Bergus, KC; Lutz, C; Aldrink, J; Bence, C; Breech, L; Dillon, PA; Downard, C; Ehrlich, PF; Fallat, M; Sato, TT; St Peter, SD; Cooper, J; Deans, K. Reducing Unnecessary Oophorectomies for Benign Ovarian Neoplasms in Pediatric Patients. Journal of the American Medical Association (JAMA). 2023; 330(13):1247-1254.

Moran-Lev, H; Kocoshis, SA; Oliveira, SB; Helmrath, M; Cole, CR. Chronic Mucosal Inflammation in Pediatric Intestinal Failure Patients-A Unique Phenomenon. Journal of Pediatric Gastroenterology and Nutrition. 2023; 77(2):e29-e35.

Oliveira, S; Moran-Lev, H; Helmrath, M; Cole, C; Kocoshis, S. 84: Inflammatory Bowel Disease-Like Findings in Pediatric Intestinal Failure Patients. Transplantation. 2023; 107(7S):50-50.

Shah, AS; Kelsey, MM; Jenkins, TM; Schaaf, LR; Xanthakos, S; Trout, AT; Baumgartner, A; Riegler, A; Krishnan, N; Bailey, A; Inge, T; Helmrath, M; Nadeau, KJ. 1115-P: Advanced Medical Therapy (AMT) for Youth-Onset Type 2 Diabetes (T2D)—Early Results from the Run-in Phase of ST2OMP. Diabetes. 2023; 72(Supplement_1).

Schaaf, LR; Crail, J; Xanthakos, S; Sizemore, J; Crimmins, NA; Helmrath, M; Ley, S; Morwessel, N; Riegler, A; Tariq, F; Shah, AS. 1105-P: Development and Implementation of a Multidisciplinary Clinic Focused on Youth-Onset Type 2 Diabetes. Diabetes. 2023; 72(Supplement_1).

Bouffi, C; Wikenheiser-Brokamp, KA; Chaturvedi, P; Sundaram, N; Goddard, GR; Wunderlich, M; Brown, NE; Staab, JF; Latanich, R; Zachos, NC; Mulloy, JC; Zorn, AM; Wells, JM; Helmrath, MA. In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice. Nature Biotechnology. 2023; 41(6):824-831.

Zeller, MH; Strong, H; Reiter-Purtill, J; Jenkins, TM; Mitchell, JE; Michalsky, MP; Helmrath, MA. Marijuana, e-cigarette, and tobacco product use in young adults who underwent pediatric bariatric surgery. Surgery for Obesity and Related Diseases. 2023; 19(5):512-521.

Singh, A; Poling, HM; Chaturvedi, P; Thorner, K; Sundaram, N; Kechele, DO; Childs, CJ; Mccauley, HA; Fisher, GW; Brown, NE; Spence, JR; Wells, JM; Helmrath, MA. Transplanted human intestinal organoids: a resource for modeling human intestinal development. Development. 2023; 150(9).

Arora, K; Sundaram, N; Ramananda, Y; Helmrath, M; Naren, AP. Applying patient personalized organoid model system to understand complex GI manifestations in cystic fibrosis disease. Physiology. 2023; 38(S1):5711443.

Gil, LA; Lutz, CM; Dillon, PA; Downard, CD; Ehrlich, PF; Fallat, ME; Fraser, JD; Grabowski, JE; Helmrath, MA; Hertweck, SP; Deans, KJ; Minneci, PC; Hewitt, GD; Aldrink, JH. Use and Accuracy of Intraoperative Frozen Section Analysis for Ovarian Masses in Children and Adolescents. Journal of Pediatric and Adolescent Gynecology. 2023; 36(2):155-159.

Marquart, JP; Bowder, AN; Bence, CM; St Peter, SD; Gadepalli, SK; Sato, TT; Szabo, A; Minneci, PC; Hirschl, RB; Rymeski, BA; Silverberg, J; Slidell, MB; Wright, TN; Lal, DR. Thoracoscopy versus thoracotomy for esophageal atresia and tracheoesophageal fistula: Outcomes from the Midwest Pediatric Surgery Consortium. Journal of Pediatric Surgery. 2023; 58(1):27-33.

Schaaf, L; Ley, S; Riegler, A; Poetker, A; Xanthakos, S; Sizemore, J; Crimmins, N; Helmrath, M; Tracy, R; Arce-Clachar, AC; Crail, J; Morwessel, N; Frenck, K; Tariq, F; Shah, AS. Development and Implementation of a Multidisciplinary Clinic Focused on the Care of Adolescents with Youth-Onset Type 2 Diabetes. Journal of Multidisciplinary Healthcare. 2023; 16:2799-2807.

Rosselot, AE; Park, M; Kim, M; Matsu-Ura, T; Wu, G; Flores, DE; Subramanian, KR; Lee, S; Sundaram, N; Broda, TR; Wells, JM; Hogenesch, JB; Moore, SR; Hong, CI. Ontogeny and function of the circadian clock in intestinal organoids. EMBO Journal. 2022; 41(2):e106973.

Fraser, JA; Deans, KJ; Fallat, ME; Helmrath, M; Kabre, R; Leys, CM; Markel, TA; Dillon, PA; Downard, C; Wright, TN; Sato, TT; Slater, BJ; Peter, SDS; Fraser, JD. Evaluating the risk of peri-umbilical hernia after sutured or sutureless gastroschisis closure. Journal of Pediatric Surgery. 2022; 57(12):786-791.

Kunisaki, SM; Saito, JM; Fallat, ME; Peter, SDS; Lal, DR; Karmakar, M; Deans, KJ; Gadepalli, SK; Hirschl, RB; Minneci, PC; Helmrath, MA. Fetal Risk Stratification and Outcomes in Children with Prenatally Diagnosed Lung Malformations: Results from a Multi-Institutional Research Collaborative. Annals of Surgery. 2022; 276(5):e622-e630.

Decker, KM; Reiter-Purtill, J; Bejarano, CM; Goldschmidt, AB; Mitchell, JE; Jenkins, TM; Helmrath, M; Inge, TH; Michalsky, MP; Zeller, MH. Psychosocial predictors of problematic eating in young adults who underwent adolescent bariatric surgery. Obesity Science & Practice. 2022; 8(5):545-555.

Pitstick, AL; Poling, HM; Sundaram, N; Lewis, PL; Kechele, DO; Sanchez, JG; Scott, MA; Broda, TR; Helmrath, MA; Wells, JM; Mayhew, CN. Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation. Stem Cell Reports. 2022; 17(8):1889-1902.

Ding, L; Chakrabarti, J; Sheriff, S; Li, Q; Thi Hong, HN; Sontz, RA; Mendoza, ZE; Schreibeis, A; Helmrath, MA; Zavros, Y; Merchant, JL. Toll-like Receptor 9 Pathway Mediates Schlafen+-MDSC Polarization During Helicobacter-induced Gastric Metaplasias. Gastroenterology. 2022; 163(2):411-425.e4.

Bowder, AN; Bence, CM; Rymeski, BA; Gadepalli, SK; Sato, TT; Szabo, A; Arendonk, KV; Minneci, PC; Downard, CD; Hirschl, RB; St Peter, SD; Troutt, M; Wright, TN; Lal, DR. Acid suppression duration does not alter anastomotic stricture rates after esophageal atresia with distal tracheoesophageal fistula repair: A prospective multi-institutional cohort study. Journal of Pediatric Surgery. 2022; 57(6):975-980.

Engwall-Gill, AJ; Chan, SS; Boyd, KP; Saito, JM; Fallat, ME; St Peter, SD; Bolger-Theut, S; Crotty, EJ; Green, JR; Hulett Bowling, RL; Mak, GZ; Minneci, PC; Wright, TN; Kunisaki, SM. Accuracy of Chest Computed Tomography in Distinguishing Cystic Pleuropulmonary Blastoma From Benign Congenital Lung Malformations in Children. JAMA Network Open. 2022; 5(6):e2219814.

Minneci, PC; Hade, EM; Gil, LA; Metzger, GA; Saito, JM; Mak, GZ; Hirschl, RB; Gadepalli, S; Helmrath, MA; Leys, CM; Kabre, R; Fallat, ME; Cooper, JN; Deans, KJ. Demographic and Clinical Characteristics Associated With the Failure of Nonoperative Management of Uncomplicated Appendicitis in Children: Secondary Analysis of a Nonrandomized Clinical Trial. JAMA Network Open. 2022; 5(5):e229712.

Arora, K; Sundaram, N; Helmrath, M; Naren, AP. Mo1117: PATIENT PERSONALIZED TRANSLATIONAL TOOLS TO STUDY GI COMPLICATIONS IN CYSTIC FIBROSIS. Gastroenterology. 2022; 162(7):s-704-s-705.

Singh, A; Poling, HM; Sundaram, N; Wells, J; O'connell, AE; Kovbasnjuk, O; Helmrath, M. 29: MESENCHYMAL WNT2B IS REQUIRED FOR MAINTENANCE OF THE HUMAN SMALL INTESTINAL EPITHELIUM. Gastroenterology. 2022; 162(7):s-11.

Dekonenko, C; Fraser, JD; Deans, K; Fallat, ME; Helmrath, M; Kabre, R; Leys, CM; Burns, RC; Corkum, K; Dillon, PA; Rymeski, B; Sato, TT; Slater, BJ; Peter, SSD. Does Use of a Feeding Protocol Change Outcomes in Gastroschisis? A Report from the Midwest Pediatric Surgery Consortium. European Journal of Pediatric Surgery. 2022; 32(2):153-159.

Capeling, MM; Huang, S; Childs, CJ; Wu, JH; Tsai, Y-H; Wu, A; Garg, N; Holloway, EM; Sundaram, N; Bouffi, C; Helmrath, M; Spence, JR. Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell reports. 2022; 38(7):110379.

Mcnelis, K; Poindexter, A; Goddard, G; Riddle, S; Wessel, J; Helmrath, M. Body Composition of Infants With Congenital Gastroschisis. Journal of Pediatric Gastroenterology and Nutrition. 2022; 74(2):e35-e38.

Chakrabarti, J; Dua-Awereh, M; Schumacher, M; Engevik, A; Hawkins, J; Helmrath, MA; Zavros, Y. Sonic Hedgehog acts as a macrophage chemoattractant during regeneration of the gastric epithelium. npj Regenerative Medicine. 2022; 7(1):3.

Eicher, AK; Kechele, DO; Sundaram, N; Berns, HM; Poling, HM; Haines, LE; Sanchez, JG; Kishimoto, K; Krishnamurthy, M; Han, L; Zorn, AM; Helmrath, MA; Wells, JM. Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells. Cell Stem Cell. 2022; 29(1):36-51.e6.