Shared Core Facilities
Research Flow Cytometry Facility

Research Flow Cytometry Facility

The Research Flow Cytometry Facility (RFCF) is a shared resource laboratory serving the Cincinnati Children's research communities and surrounding institutions by providing a central location for flow cytometry instrumentation, training and education. We offer a number of services for both internal and external scientists.

New users need to be trained for their instrument of choice. The training depends on the instrument needed. See Education and Training for more information.

If you have any questions, please contact Celine Silva Lages.

High Parameter Meeting Dates 

Details Coming Soon

ORVCA Monthly User Meeting Dates 
1-2 PM

Date  Room  Presenter Subject
8/28/2024  S5.125

Celine Silva Lages 
RFCF Manager

 RFCF update: website, policies and Stratocore
9/4/2024  S6.125; 9-1 PM David Morris 
BD Biosciences
 BD THNK Spectral Education Series
9/25/2024  S9.130 Chris Langsdorf
Thermofisher 
 TBD
10/23/2024  S6.125 Rathnakumar Kumaragurubaran
Assistant Professor
 Xenium update
10/30/2024  TBD  TBD  Cytorecovery
11/20/2024  Online  TBD  Imaris
12/18/2024 S6.125
 TBD  TBD

Children’s employees: From the lobby of the S building, take an elevator to the 5th floor. Turn left out of the elevator bay and proceed to the R building. Our desks are located in R5503 and the office is in R5564. The instrument rooms are R5503, R5504, R5509, R5510, R5524, R5526, R5529, R5543 and R5087A. From the R building, take an elevator to the 5th floor. Turn left and then right and proceed down the hallway to our area (see room numbers above).

Non-Children’s employees: If you are driving to Cincinnati Children’s, please view directions, maps and information about parking. See building and room numbers above.

When publishing data that is generated using our facility, it is necessary to properly acknowledge us in your publications. See below for example statements and please acknowledge specific staff members as appropriate.

To acknowledge our assistance:
We would like to acknowledge the assistance of the Research Flow Cytometry Facility in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center.

To acknowledge our facility:
All flow cytometric data were acquired using equipment maintained by the Research Flow Cytometry Facility in the Division of Rheumatology at Cincinnati Children’s Hospital Medical Center.

For NIH P30 Center Members:
If you are a member of a NIH P30 grant at Cincinnati Children’s, please include ONLY the P30 grant number of the corresponding center by adding the following statement in the acknowledgements ‘supported in part by insert appropriate P30 grant reference(s) here’ (see below).

1) Center for Rheumatic Disease Research (NIH AR070549) or 2) Digestive Health Center (NIDDK P30 DK078392).

For users of the SH800S cell sorter:

For data generated using the SH800S cell sorter please acknowledge the grant used to purchase the instrument by adding the following statement in publications: “supported by NIH S10OD023410”.

For users of the Aurora analyzer:

For data generated using the Aurora analyzer please acknowledge the grant used to purchase the instrument by adding the following statement in publications: “supported by NIH S10OD025045”.

 

General Information

Scheduling:

Analyzers and Sorters: Stratocore

Location

Office: R5503 (Lab desks), R5564 (Main Office)

Analyzer rooms:
R5087A: FACSCantos 1, 2 and 3
R5524: LSRFortessas 1, 2 and 3; and FACSymphony A5
R5503: ImageStreamX Mark II
R5509: Auroras

Sorting Rooms:
R5503: FACSArias and FACSymphony S6
R5504: MA900 and SH800
R5529: BigFoot

Luminex/ELISA:
R5503

IsoLight:
R5526

Cellometer Vision:
R5503

EnVision:
R5522

GentleMACS Dissociator:
R5510

Analyzer Hours
24 / 7 for those who have been trained by our staff

Sorting Hours
9:30 am to 5:30 pm Monday-Friday (Staff operated)

Contact Us
General information, help and analyzer training:
513-636-3575

Cell sorter rooms:
513-636-2770 - FACSArias and FACSymphony S6
513-636-2054 - BigFoot
513-517-1466 - SH800 and MA900

Analyzer rooms:
513-636-3332