Protocol for establishing inducible CRISPR interference system for multiple-gene silencing in human pluripotent stem cells. Matsui, S; Shiley, JR; Granitto, M; Ludwig, K; Buckley, M; Koigi, S; Mirizio, G; Hu, YC; Mayhew, CN; Iwafuchi, M. Star Protocols. 2024; 5:103221.

Protocol for establishing inducible CRISPRd system for blocking transcription factor-binding sites in human pluripotent stem cells. Matsui, S; Shiley, JR; Buckley, M; Lim, HW; Hu, YC; Mayhew, CN; Iwafuchi, M. Star Protocols. 2024; 5:103233.

Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon. Múnera, JO; Kechele, DO; Bouffi, C; Qu, N; Jing, R; Maity, P; Enriquez, JR; Han, L; Campbell, I; Mahe, MM; Hu, YC; Takebe, T; Helmrath, MA; Wells, JM. Cell Stem Cell. 2023; 30:1434-1451.e9.

iPSC-derived neural precursor cells engineering GBA1 recovers acid β-glucosidase deficiency and diminishes α-synuclein and neuropathology. Peng, Y; Liou, B; Lin, Y; Mayhew, CN; Fleming, SM; Sun, Y. Molecular Therapy-Methods & Clinical Development. 2023; 29:185-201.

A review of protocols for brain organoids and applications for disease modeling. Mayhew, CN; Singhania, R. Star Protocols. 2023; 4:101860.

Implementing Research Shared (Core) Facility Billing Systems. Chimote, AA; Mayhew, C; Thornton, S; Wetzel, C; White, AN. Journal of biomolecular techniques : JBT. 2022; 33.

Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation. Pitstick, AL; Poling, HM; Sundaram, N; Lewis, PL; Kechele, DO; Sanchez, JG; Scott, MA; Broda, TR; Helmrath, MA; Wells, JM; Mayhew, CN. Stem Cell Reports. 2022; 17:1889-1902.

Human iPSC-derived hepatocyte system models cholestasis with tight junction protein 2 deficiency. Li, CZ; Ogawa, H; Ng, SS; Chen, X; Kishimoto, E; Sakabe, K; Fukami, A; Hu, YC; Mayhew, CN; Hellmann, J; Hayashi, H; Thompson, RJ; Rashid, ST; Asai, A. JHEP Reports. 2022; 4:100446.

High-Throughput Functional Analysis of CFTR and Other Apically Localized Proteins in iPSC-Derived Human Intestinal Organoids. Xia, S; Bozóky, Z; Di Paola, M; Laselva, O; Ahmadi, S; Jiang, JX; Pitstick, AL; Jiang, C; Rotin, D; Mayhew, CN; Jones, NL; Bear, CE. Cells. 2021; 10.

Modeling Human Bile Acid Transport and Synthesis in Stem Cell-Derived Hepatocytes with a Patient-Specific Mutation. Hayashi, H; Osaka, S; Sakabe, K; Fukami, A; Kishimoto, E; Aihara, E; Sabu, Y; Mizutani, A; Kusuhara, H; Naritaka, N; Mayhew, C; Setchell, K; Takebe, T; Asai, A. Stem Cell Reports. 2021; 16:309-323.