Helping Develop and Implement Protocols for the Effective Differentiation of hPSCs

A key objective of the Pluripotent Stem Cell Facility is to assist in both the implementation of existing protocols and the development of new protocols for the effective differentiation of hPSCs to cell types useful for basic and translational research studies.

We offer hands-on training in directed differentiation of hPSCs to human small intestinal organoids (HIOs), human colonic organoids (HCOs), and human antral gastric organoids (aHGOs).

We also developed an improved protocol for generation of small intestinal organoids. This protocol relies on the generation of cryopreservable mid-hindgut endoderm (MHE) that can be thawed and subsequently aggregated for robust and reproducible HIO and HCO generation.

Our facility provides access to a validated cryopreserved bank of WA01/H1-derived MHE, and offers a service to generate a bank of MHE from any hPSC line.

These services are available to Cincinnati Children's and the University of Cincinnati researchers. For more information, contact us.